Chemically Defined Culture and Cardiomyocyte Differentiation of Human Pluripotent Stem Cells
نویسندگان
چکیده
منابع مشابه
Cardiomyocyte differentiation of human induced pluripotent stem cells.
BACKGROUND The ability to derive human induced pluripotent stem (hiPS) cell lines by reprogramming of adult fibroblasts with a set of transcription factors offers unique opportunities for basic and translational cardiovascular research. In the present study, we aimed to characterize the cardiomyocyte differentiation potential of hiPS cells and to study the molecular, structural, and functional ...
متن کاملMARI PEKKANEN - MATTILA Cardiomyocyte Differentiation from Human Pluripotent Stem Cells
................................................................................................................5 Tiivistelmä.............................................................................................................7 Table of contents ...................................................................................................9 List of original publications ...............
متن کاملDownregulation of LGR5 Expression Inhibits Cardiomyocyte Differentiation and Potentiates Endothelial Differentiation from Human Pluripotent Stem Cells
Understanding molecules involved in differentiation of human pluripotent stem cells (hPSCs) into cardiomyocytes and endothelial cells is important in advancing hPSCs for cell therapy and drug testing. Here, we report that LGR5, a leucine-rich repeat-containing G-protein-coupled receptor, plays a critical role in hPSC differentiation into cardiomyocytes and endothelial cells. LGR5 expression was...
متن کاملdifferentiation of definitive endoderm from human induced pluripotent stem cells on hmscs feeder in a defined medium
background: the definitive endoderm (de) differentiation using the undefined media and non-human feeders can cause contaminations in the generated cells for therapeutic applications. therefore, generating safer and more appropriate de cells is needed. this study compared five different methods to establish an appropriate method for inducing an efficient de differentiation from human induced plu...
متن کاملA fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation.
Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced pluripotent stem cells, are promising for numerous biomedical applications, such as cell replacement therapies, tissue and whole-organ engineering, and high-throughput pharmacology and toxicology screening. Each of these applications requires large numbers of cells of high quality; however, the scalable expan...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols in Human Genetics
سال: 2015
ISSN: 1934-8266,1934-8258
DOI: 10.1002/0471142905.hg2103s87